LB Agar

Tryptone 10 g
Yeast extract 5 g
Sodium chloride 5 g
Water To 1 litre
  • pH to 7.5 with NaOH
  • Add 15g agarose just before autoclaving.
  • Autoclave.Allow medium to cool to 50oC before adding ampicillin to a final concentration of 100 ug/ml (1/1000 dilution of a 100 mg/ml ampicillin stock solution). Plates can be stored for 1 month at 4oC or 1 week at room temperature.
Sham Nair 2014