SDS-PAGE AND COOMASSIE BLUE STAINING

SDS-polyacrylamide gels (10%) were loaded with 20 μg protein/lane (10 μg proteins/lane for Western blotting) and separated for an initial 10 min at 100 V followed by 80 min at 150 V and stained with Coomassie blue silver stain (20% methanol, 10% (w/v) ammonium sulphate, 1.6% orthophosphoric acid, 0.15% (w/v) Coomassie G250). Either Broad Range or Precision Plus protein markers (Bio-Rad) were used as size standards.

Sham Nair 2014